Sterilize by autoclaving at 15 lbs. Bile esculin agar (BEA) is a differential and selective medium and is mainly used to discern group D Streptococci and Enterococci based on the organism’s potential to hydrolyze esculin. Cool to 45-50°C and pour the mediia from the erlenmeyer flasks into plates. However, other tests such as salt tolerance should be performed for identifying Enterococci ( 3). Bile Esculin Agar was also shown to aid differentiation of Enterobacteriaceae, Klebsiella, Enterobacter, Serratia from other Enterobacteriaceae genera (11) on the basis of esculin hydrolysis. This formula modifies Bile Esculin Agar by adding sodium azide and reducing the concentration of bile. II PERFORMANCE TEST PROCEDURE 1. II PERFORMANCE TEST PROCEDURE 1. Heat to boiling to dissolve the medium completely. BD BBL™ Bacteroides Bile Esculin Agar is a selective medium for the isolation and presumptive identification of the group. Preparation Instructions Suspend 63.5 grams of Bile Esculin Agar Base in 1000 mls of distilled water. Bile Aesculin Agar is designed to differentiate between Group D streptococci and non-Group D streptococci.. Group D streptococci hydrolyse aesculin to form aesculetin and dextrose.Aesculin hydrolysis is indicative of a positive result. Reduce all BBE plates overnight at room temperature in a BD GasPak™ EZ anaerobic system. Meyer and Schonfeld incorporated bile into the esculin medium and showed that 61 of 62 enterococci were able to grow and split esculin, whereas the other streptococci could not. Typical formula (g/l)* Tryptone 17.0; Peptone 3.0; Yeast extract 5.0; Ox bile 10.0; Sodium chloride 5.0; Esculin 1.0… pressure (121°C) for 15 minutes. Add 1 gram of E8250 Esculin and mix. 2. Isolate and presumptively identify enterococci / Group D streptococci with Thermo Scientific™ Oxoid™ Bile Aesculin Agar (Dehydrated). Reduce all BBE plates overnight at room temperature in a BD GasPak™EZ anaerobic system. The medium is highly nutritious. Bacteroides Bile Esculin Agar is a selective medium for the isolation and presumptive identification of the B. fragilisgroup. 2. The revised medium is more selective, but still provides rapid growth and efficient recovery of group D streptococci. Bile Esculin Azide Agar is a modification of the medium reported by Isenberg and Isenberg, Goldberg, and Sampson. V SUMMARY AND EXPLANATION Rochaix noted the value of esculin hydrolysis in the identification of enterococci.1 The enterococci were able to split esculin, but other streptococci could not. Intended use The selective isolation and differentiation of enterococci. Dispense in tubes or erlenmeyer flasks. Swan used an esculin medium containing 40% bile salts and reported that a positive reaction on the bile esculin medium correlated with a serological group D precipitin reaction. Preparation of inocula a. Meyer and Schonfeld incorporated bile into the esculin medium and showed … Enterococcosel Agar, a modified esculin bile agar, is used for the rapid, selective detection and enumeration of enterococci. Preparation of inocula a.
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